Regulatory

Part:BBa_K1314000:Experience

Designed by: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting   Group: iGEM14_Hong_Kong-CUHK   (2014-10-06)

EcoRI PstI double digest check the gel photo here shows the expected band size of 250 bp of insert: nifH promoter

Applications of BBa_K1314000

Expressing nifH promoter(Bba_K1314000) with BBa_E1010 in E. coli DH5a

Bba_K1314001 is comprised of nifH promoter and Bba_E1010. It is designed for testing nifH promoter. The right tube shows the expression of RFP using ptep promoter in Dh5a and the left tube show the expression of BbaK1314001 in DH5a. The result show that nifH promoter is not working in DH5a. NifH E1010.jpg

Characterization of Bba_K1314001, Bba_K1314013, Bba_K1314014 in Azotobacter vinelandii strain DJ

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User Reviews

It is a promoter designed for Azotobacter vinelandii. It is not functional in E. coli. UNIQab3ebd10b79cdc9e-partinfo-00000001-QINU UNIQab3ebd10b79cdc9e-partinfo-00000002-QINU